Understanding:
• Marker genes are used to indicate successful uptake
Following gene delivery, scientists must identify which cells have successfully taken up the recombinant DNA
- These transgenic cells can then be cultured and developed to form transgenic crops for agricultural use
Marker genes are typically included as part of the recombinant construct to indicate successful uptake
- Most commonly, marker genes will code for resistance to a particular antibiotic
- Plant cells are grown on media containing the antibiotic and only the transgenic cells will be able to survive
Marker Genes